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PMC:1064895 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor ?B-depend 34 2395 0 show
1 Introduction Rheumatoid arthritis (RA) is characterized by infiltrations of macrophages and T cells into the join 27 3227 0 show
2 Materials and methods Patients Informed consent was obtained from 24 patients (5 men and 19 women) with RA who fulfilled t 27 783 0 show
3 Materials and methods Reagents Recombinant IL-17, IL-18, IL-15, monocyte chemoattractant protein-1 (MCP-1), macrophage inf 27 832 0 show
4 Materials and methods Production of IL-17 by T cell receptor activation, cytokines or chemokines PBMC were prepared from h 27 1029 0 show
5 Materials and methods CD4+ T-cell isolation by MACS Anti-CD4 microbeads were used essentially as recommended by the manufa 27 1096 0 show
6 Materials and methods Enzyme-linked immunosorbent assay of IL-17 IL-17 in culture supernatants was measured by sandwich en 27 1480 0 show
7 Materials and methods Quantification of IL-17 mRNA by semiquantitative reverse transcription–polymerase chain reaction PBM 27 1592 0 show
8 Materials and methods Western blot analysis of Akt, phosphorylated Akt and IκB-α PBMC were incubated with anti-CD3 (10 μg/ 27 1256 0 show
9 Materials and methods Gel mobility-shift assay of NF-κB binding site Nuclear proteins were extracted from about 5 × 106 PB 27 1146 0 show
10 Materials and methods Cell viability (Trypan blue dye exclusion assay) For cell viability assays, the trypan blue dye excl 27 394 0 show
11 Materials and methods Statistical analysis Data are expressed as means ± SEM. Statistical analysis was performed with Stud 27 184 0 show
12 Results IL-17 production in PBMC from patients with RA, patients with OA and normal individuals PBMC were se 27 625 0 show
13 Results Increased IL-17 production in PBMC of patients with RA by anti-CD3 and/or anti-CD28, and PHA Because 27 848 0 show
14 Results Regulation of IL-17 production in RA PBMC by inflammatory cytokines and chemokines Because RA PBMC i 27 931 0 show
15 Results Inhibition of IL-17 production by signal transduction inhibitors and anti-rheumatic drugs Having obs 27 1429 0 show
16 Results IL-17 mRNA expression in RA PBMC To see whether enhanced IL-17 production could be regulated at a tr 27 390 0 show
17 Results Activation of PI3K/Akt signal transduction pathway on IL-17 production by anti-CD3 To determine down 27 829 0 show
18 Results Activation of the NF-κB and activator protein-1 (AP-1) pathway in the IL-17 promoter region To inves 27 1434 0 show
19 Discussion IL-17 was first described as a T cell product with proinflammatory properties [5,22]. RA is characte 27 6083 0 show
20 Conclusions We have detected a more pronounced production of IL-17 from RA PBMC in response to IL-15 and MCP-1 a 27 695 0 show