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Divisions (32)
 

div. section text # proj. length # Ann.
0 TIAB The Transcription Factor C/EBP-β Mediates Constitutive and LPS-Inducible Transcription of Murine Ser 33 1764 1048 show
1 Introduction The inflammatory response is a double-edged sword. Properly orchestrated, it results in the clearing 26 3529 2054 show
2 Experimental Procedures Cell Culture Murine macrophage RAW 264.7 cells (ATCC TIB-71) were maintained in RPMI 1640 media (Gib 26 1317 414 show
3 Experimental Procedures Real-time Quantitative PCR (qPCR) The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from ce 26 405 231 show
4 Experimental Procedures DNA Sequence Analysis The DNA sequence of the murine SerpinB2 promoter was determined by sequencing 26 1154 441 show
5 Experimental Procedures Western Blot Assays Whole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Trit 26 772 409 show
6 Experimental Procedures Construction of SerpinB2 Reporter Gene Plasmids The PCR primers DW5’LUC, containing a Kpn I restrict 26 2230 1239 show
7 Experimental Procedures Transient Transfection and Luciferase Assays (RAW264.7 Macrophages) RAW 264.7 cells (2×107) growing 26 1323 683 show
8 Experimental Procedures Lentiviral shRNAs, Packaging and Transduction pLKO.1-puro lentiviral vectors carrying short hairpin 26 1192 533 show
9 Experimental Procedures Transient Transfection and Luciferase Assays (MEF Cells) Cebpb−/− MEFs (1×105) [34] were transfected 26 1042 652 show
10 Experimental Procedures Electrophoretic Mobility Shift Assays Radiolabelled, double-stranded oligonucleotide probes for gel 26 942 441 show
11 Experimental Procedures Chromatin Immunoprecipitation (ChIP) Assay ChIP assays were performed using a commercially available 26 1451 643 show
12 Experimental Procedures Statistical Analysis Data are presented as mean ± SEM per group. Results were analyzed using the ana 26 205 77 show
13 Results The SerpinB2 Gene is Highly Responsive to LPS When RAW264.7 macrophages were exposed to LPS, SerpinB 26 675 438 show
14 Results The SerpinB2 Proximal Promoter Confers LPS Responsiveness To investigate cis-acting regulatory eleme 26 1737 910 show
15 Results Sequence Conservation within the Human and Murine SerpinB2 Proximal Promoters To look for potential 26 1928 1040 show
16 Results A C/EBP, CRE and Two AP-1 Sites in the Murine SerpinB2 Gene Proximal Promoter are Essential for Opti 26 1749 972 show
17 Results The C/EBP Element is Bound by an LPS-induced Nuclear Factor from RAW 264.7 Macrophages Nuclear facto 26 1472 707 show
18 Results C/EBP-β is a LPS-induced Nuclear Factor that Binds to the C/EBP Element of the SerpinB2 Proximal Pro 26 1268 741 show
19 Results C/EBP-β Mediates both Constitutive and LPS-induced SerpinB2 mRNA Expression in MEFs and Inflammatory 26 1193 920 show
20 Results C/EBP-β Binds the Murine SerpinB2 Proximal Promoter in vivo in an LPS-inducible Manner We investigat 26 1653 854 show
21 Results C/EBP-β Promotes LPS-inducible Murine SerpinB2 Proximal Promoter Activity Since C/EBP-β binds to the 26 802 617 show
22 Results Phosphorylation of C/EBP-β at Serine 64 negatively Regulates LPS-stimulated SerpinB2 Promoter Activi 26 2244 1789 show
23 Discussion Macrophages are key mediators of the innate immune response, and consequently provide the first line 26 6643 4205 show
24 Caption-Figure 1 LPS induction of SerpinB2 mRNA and protein expression in murine macrophage cells. RAW264.7 macrophag 26 450 283 show
25 Caption-Figure 2 Deletion reporter gene analysis of LPS and PMA-responsive regions in the murine SerpinB2 promoter. ( 26 1515 702 show
26 Caption-Figure 3 Potential cis-acting regulatory elements in the LPS responsive region of the murine SerpinB2 promote 26 661 385 show
27 Caption-Figure 4 Identification of cis-acting elements required for the LPS-responsiveness of the murine SerpinB2 pro 26 814 380 show
28 Caption-Figure 5 The LPS-inducible nuclear factor binding the murine SerpinB2 proximal promoter C/EBP site contains C 26 860 350 show
29 Caption-Figure 6 C/EBP-β is essential for constitutive and LPS-induced SerpinB2 mRNA expression. (A) Endogenous Serpi 26 1169 749 show
30 Caption-Figure 7 Differential recruitment of C/EBP-β and C/EBP-βT217 to the murine SerpinB2 promoter in vivo. Transcr 26 1146 694 show
31 Caption-Figure 8 C/EBP-β is necessary for SerpinB2 proximal promoter activity. (A) The SerpinB2 proximal promoter is 26 1430 1027 show