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Divisions (30)
 

div. section text # proj. length # Ann.
0 TIAB Regulation ofMycobacterium tuberculosis-Dependent HIV-1 Transcription Reveals a New Role for NFAT5 i 32 1450 823 show
1 Introduction Mycobacterium tuberculosis (MTb), the causative agent of tuberculosis (TB), is the most common co-in 25 3580 1953 show
2 Materials and Methods Ethics statement In our studies we used unidentified human discarded blood cells (peripheral blood m 25 196 79 show
3 Materials and Methods Cell culture PBMC from normal unidentified donors were isolated by Ficoll-Hypaque (Pharmacia Corpora 25 1363 575 show
4 Materials and Methods Viruses HIV-1Bal, HIV-1Lai, HIV-193TH64, HIV-192TH51, HIV-192TH53, HIV-198CH01, and HIV-198IN22 were 25 305 108 show
5 Materials and Methods LTR plasmid construction and reporter assay LTR reporter plasmids were constructed by inserting nucl 25 1179 483 show
6 Materials and Methods Quantitative DNase I footprinting Recombinant NFAT5 (amino acids 175–471) with an N-terminal 6× His 25 399 243 show
7 Materials and Methods HIV-1 infectious molecular clones The plasmid encoding the full-length infectious molecular clone of 25 794 318 show
8 Materials and Methods siRNA transfection of MDM An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequenc 25 838 407 show
9 Materials and Methods Stable THP-1 cells expressing shRNA The lentiviral plasmid pLKO.1 expressing shRNA targeting human M 25 1452 554 show
10 Materials and Methods MTb culture The MTb clinical strain CDC1551 was prepared by adding 100 µl of frozen bacteria stock i 25 949 385 show
11 Materials and Methods Western blot Whole cell extracts were collected with lysis buffer containing 150 mM NaCl, 50 mM Tris 25 1149 536 show
12 Materials and Methods Quantitative PCR The mRNA expression levels were determined by SYBR Green-based real-time PCR (Appli 25 352 152 show
13 Materials and Methods Statistical analysis Where applicable, results are expressed as mean ± SEM. Comparison between two g 25 228 78 show
14 Results MTb increases HIV-1 LTR activity of HIV-1 subtypes B, C, and E To compare the functional impact of M 25 1083 498 show
15 Results Specific disruption of NFAT5 binding impairs LTR-driven transcription in THP-1 cells in response to 25 1481 827 show
16 Results MTb increases NFAT5 mRNA levels in human MDM Given that macrophages, which are the primary target of 25 1115 654 show
17 Results RNAi directed against NFAT5 inhibits replication of subtype B HIV-1 To extend the results we obtaine 25 2792 1505 show
18 Results NF-κB and NFAT5 binding to the HIV-1 LTR can be specifically disrupted To demonstrate that the impac 25 2672 1848 show
19 Results NF-κB and NFAT5 binding play independent activating roles in MTb-mediated induction of HIV-1 replica 25 4282 1500 show
20 Results NFAT5 is important for enhancement of HIV-1 subtype C replication in response to MTb infection As sh 25 1789 792 show
21 Results MTb induces NFAT5 gene expression via the MyD88-dependent signaling cascade To begin to decipher the 25 3114 2432 show
22 Discussion The transcription factor NFAT5 is the most evolutionarily divergent member of the Rel family of NFAT 25 7007 3977 show
23 Caption-Figure 1 NFAT5 interaction with the LTR is important for MTb-induced HIV-1 transcription. (A) MTb stimulation 25 2854 1313 show
24 Caption-Figure 2 MTb induces NFAT5 gene expression and knockdown of NFAT5 impairs HIV-1 replication during MTb co-inf 25 2152 1259 show
25 Caption-Figure 3 Specific disruption of NFAT5 or NF-κB binding sites in the LTR of HIV-1 subtype B. (A) Mutations int 25 2236 1606 show
26 Caption-Figure 4 MTb-induced HIV-1 replication in PBMC depends on binding of NFAT5 and NF-κB to the LTR. Specific dis 25 1002 578 show
27 Caption-Figure 5 Disruption of NF-κB or NFAT5 sites in the LTR inhibits MTb-induced HIV-1 replication in MDM. MDM fro 25 702 454 show
28 Caption-Figure 6 Disruption of the NFAT5 site in HIV-1 subtype C significantly impairs MTb-induced viral replication. 25 1146 519 show
29 Caption-Figure 7 The MyD88-mediated signaling cascade is important for MTb induction of NFAT5 expression in human mon 25 2036 1526 show