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Divisions (33)
 

div. section text # proj. length # Ann.
0 TIAB Incompetence of Neutrophils to Invasive Group AstreptococcusIs Attributed to Induction of Plural Vir 1 1988 29 show
1 Introduction Streptococcus pyogenes (group A streptococcus; GAS) is one of the most common human pathogens. It ca 1 3345 44 show
2 Results Group A streptococcus isolates from severe invasive infections is resistant to killing by human PMN 1 1040 14 show
3 Results Severe invasive GAS rapidly induce necrosis to human PMN In an acute bacterial infection, PMN were q 1 1227 12 show
4 Results PMN were killed by streptolysin O (SLO) from severe invasive GAS PMN death was induced shortly after 1 2106 57 show
5 Results Impaired migration of PMN is due to degradation of IL-8 by serine proteinase ScpC Although NIH230slo 1 1375 38 show
6 Results Enhanced expression of the slo and the scpC genes in severe invasive GAS is attributed to mutation o 1 3456 80 show
7 Results csrS mutation is important in the pathogenesis of invasive infections in a mouse model In order to e 1 1831 42 show
8 Results scpC and slo are insufficient singly for the pathogenesis of invasive infections Finally, we assesse 1 718 21 show
9 Discussion It have been demonstrated that CsrS/R is a member of the two-component regulatory systems for regula 1 7839 121 show
10 Methods Bacterial strains and culture The S. pyogenes strains used in this study are described in Table S1 [ 1 817 0 show
11 Methods Animals Male 5–6-week-old outbred ddY and hairless mice were purchased from SLC (Shizuoka, Japan) an 1 338 0 show
12 Methods Isolation of human PMN PMN were taken from nine healthy volunteers which were composed of 25–52 year 1 314 0 show
13 Methods DNA manipulation DNA amplifications by PCR, DNA restriction-endonuclease digestions, ligations, plas 1 415 0 show
14 Methods Transformation Calcium chloride (CaCl2) competent E. coli cells were prepared and transformed accord 1 200 0 show
15 Methods Construction of deletion or deficient mutants Construction of the csrS mutants. A 1002-bp DNA fragme 1 3452 0 show
16 Methods Construction of the strains integrating the intact csrS gene The csrS gene replacement was performed 1 788 0 show
17 Methods RT-PCR S. pyogenes was grown in THY media at 37°C without aeration, and total RNA was extracted at O 1 350 0 show
18 Methods GAS infection in a mouse model To determine LD50, We injected several dilutions of 0.5 mL of GAS iso 1 735 0 show
19 Methods Histopathology For histological analysis, the tissues from GAS-infected mice were fixed in 10% forma 1 245 0 show
20 Methods Phagocytosis and killing assay Phagocytosis and killing assay by PMN were performed as previously de 1 946 0 show
21 Methods Migration assay Chemotaxis assay were performed as previously described with modification [42]. Brie 1 874 0 show
22 Methods ELISA The amount of IL-8 in supernatant after incubation with bacteria was determined by Ready-to-Go 1 189 0 show
23 Methods SLO-hemolysis assay The activity of SLO in supernatant is measured as previously described [43]. Bri 1 886 0 show
24 Methods SLS-hemolytic assay Overnight culture of various strains were frozen at −80°C, thawed, and centrifug 1 689 0 show
25 Caption-Figure 1 Severe invasive GAS evade killing activity of PMN. (A) Phagocytosis activity of PMN to emm49 GAS. Fo 1 564 0 show
26 Caption-Figure 2 Severe invasive GAS kill human PMN and impair migration ability in response to IL-8. (A) Flow cytome 1 1114 0 show
27 Caption-Figure 3 Severe invasive GAS killed PMN by SLO in a contact-dependent manner. (A) The viability of viable PMN 1 1473 0 show
28 Caption-Figure 4 Severe invasive GAS degrade IL-8 by serine protease ScpC, resulting in impaired PMN migration. (A) M 1 690 0 show
29 Caption-Figure 5 Mutation of the csrS gene in the isolates of the patients with severe invasive infections is respons 1 1205 0 show
30 Caption-Figure 6 Mutations of CsrS is responsible for increased virulence of GAS to PMN functions. (A) Non-invasive, 1 927 0 show
31 Caption-Figure 7 Mutations of the csrS, SLO and ScpC regulate in vivo virulence of GAS in a mouse model. (A) Number o 1 942 0 show
32 Caption-Table 1 LD50 values of each strain. 1 27 0 show