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PMC:2430206 JSONTXT

Divisions (15)
 

div. section text # proj. length # Ann.
0 TIAB The GraRS regulatory system controlsStaphylococcus aureussusceptibility to antimicrobial host defens 1 1177 29 show
1 Background Staphylococcus aureus, a frequent cause of human infections, is highly resistant to antimicrobial fa 1 2117 35 show
2 Results The graRS mutant is more susceptible to killing by LL-37 and human neutrophil granulocytes in vitro 1 1473 44 show
3 Results Deletion of graRS leads to attenuated virulence in a mouse infection model To investigate the impact 1 838 19 show
4 Discussion A large variety of regulatory systems has been described in S. aureus during the last decades [16-18 1 2113 0 show
5 Conclusion We could show here that, besides its role in influencing the effectiveness of pharmacologic antimicr 1 371 0 show
6 Methods Strains and growth conditions Staphylococcus aureus SA113 (WT), the isogenic graRS deletion mutant [ 1 981 0 show
7 Methods Cytochrome c binding assay Log-phase bacteria were harvested, washed twice with potassium phosphate 1 702 0 show
8 Methods Lipid analyses Comparison of membrane lipid patterns by extraction of polar lipids and subsequent th 1 165 0 show
9 Methods Inactivation assay with human defense peptide LL-37 Log-phase bacteria were harvested, washed twice 1 515 0 show
10 Methods Inactivation assay with human neutrophil granulocytes Bacteria were grown to logarithmic phase, wash 1 880 0 show
11 Methods Mouse infection model All procedures involving animals were approved by the UCSD Animal Care Committ 1 846 0 show
12 Caption-Figure 1 Binding of cationic cytochrome c (cyt c) by S. aureus Sa113 wild type, graRS mutant, and complemente 1 462 0 show
13 Caption-Figure 2 (A) Inactivation of wild type (black bar), graRS mutant (white bar) and complemented mutant (grey ba 1 523 0 show
14 Caption-Figure 3 CFU of wild-type and mutant bacteria in kidneys of mice 72 h after intraperitoneal infection with 1. 1 287 0 show