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Divisions (23)
 

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0 TIAB Sp1 and Sp3 regulate basal transcription of the human APOBEC3G gene APOBEC3G (A3G), a member of the 29 1411 700 show
1 INTRODUCTION The recently discovered APOBEC3 family of cytidine deaminases is considered to play an important rol 21 2921 1608 show
2 MATERIALS AND METHODS Cell culture The human T cell lines A3.01 and PM1 (NIBSC, UK) and the human myeloid cell line U937 ( 21 706 279 show
3 MATERIALS AND METHODS Plasmids For cloning of an APOBEC3G promoter-driven reporter plasmid, genomic DNA was prepared from 21 5401 1272 show
4 MATERIALS AND METHODS 5′-Rapid amplification of cDNA ends analysis (RACE) Total RNA was isolated from A3.01 T cells using 21 670 265 show
5 MATERIALS AND METHODS Luciferase assay For transient transfection of A3.01 and U937 cells, DMRIE-C transfection reagent (L 21 1877 820 show
6 MATERIALS AND METHODS Electrophoretic mobility shift assay (EMSA) For preparation of nuclear extracts, 5 × 106 A3.01 T cel 21 2293 981 show
7 MATERIALS AND METHODS Chromatin immunoprecipitation (ChIP) assay A3.01 cells were treated with RPMI culture medium contain 21 1967 886 show
8 MATERIALS AND METHODS RNA interference and western blot analysis Sp1 and Sp3 translation was silenced in HeLa cells using 21 1283 653 show
9 RESULTS Characterization of the transcriptional start sites of APOBEC3G by 5′-RACE To identify the transcrip 21 1031 471 show
10 RESULTS The core promoter of A3G is located within the region −114/+66 relative to the TSS For characterizat 21 1559 722 show
11 RESULTS The A3G promoter is not inducible in T cells According to the current knowledge, APOBEC3G plays a ro 21 2859 1574 show
12 RESULTS A GC-box located at position −87/−78 of the A3G promoter is important for transcriptional activity T 21 2018 1035 show
13 RESULTS Sp1 and Sp3 transcription factors bind to the GC-box at position −87/−78 of the A3G promoter To inve 21 2607 1536 show
14 RESULTS Silencing of Sp1 and Sp3 reduces A3G promoter activity To confirm the role of Sp1 and Sp3 in regulat 21 1045 677 show
15 DISCUSSION Shortly after the discovery of APOBEC3G, it became clear that this human gene plays an important rol 21 8562 4854 show
16 Caption-Figure 1. Sequence of the A3G promoter and the downstream region. The first 1000 bp upstream of the major TSS 21 744 335 show
17 Caption-Figure 2. 5′-RACE analysis of the A3G cDNA. Agarose gel electrophoresis of size marker and A3G 5′-RACE product 21 263 120 show
18 Caption-Figure 3. Luciferase activities of A3G promoter constructs in different cell lines. (A) A3G promoter 5′ deleti 21 852 497 show
19 Caption-Figure 4. A3G promoter activities after coexpression of HIV-1 proteins or treatment with TPA or interferons. ( 21 1581 876 show
20 Caption-Figure 5. A GC-box mediates transcriptional activity of the 180 bp core promoter. (A) A3.01 T cells were trans 21 861 472 show
21 Caption-Figure 6. Sp1 and Sp3 bind to the GC-box present in the A3G promoter. (A) Nuclear extracts of A3.01 T cells we 21 1173 748 show
22 Caption-Figure 7. Silencing of Sp1 and Sp3 reduces A3G promoter activity. (A) HeLa cells were transfected with 150 and 21 808 568 show