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Divisions (19)
 

div. section text # proj. length # Ann.
0 TIAB BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of 25 1387 955 show
1 Background Members of the transforming growth factor β (TGF-β) superfamily play central roles in controlling ce 19 2614 1599 show
2 Results BMP-6 inhibits anti-IgM induced proliferation of human B cells The effects of BMP-6 on normal and ne 19 1731 999 show
3 Results BMP-6 induces cell death in human memory B cells and Ramos cells Next, we wanted to establish whethe 19 728 438 show
4 Results Human B cells express BMP-6 receptors Detailed knowledge regarding expression of different BMP recep 19 806 524 show
5 Results BMP-6 induces phosphorylation of Smad1/5/8 Upon ligand binding, the type II receptor transphosphoryl 19 1168 775 show
6 Results BMP-6 induces upregulation of Id1 Next, we wanted to explore whether the BMP-6 induced phosphorylati 19 1714 1123 show
7 Results BMP-6 production in B cells The fact that BMP-6 has been reported to act as an autocrine stimulator 19 1310 707 show
8 Discussion Recent studies have demonstrated an important role for BMP superfamily members in hematopoietic stem 19 10637 6255 show
9 Conclusion In conclusion, our results show that BMP-6 induces activation of intracellular Smad signalling in ma 19 610 316 show
10 Methods Cell culture If not specified, all cells were cultured in X-VIVO 15™ (BioWhittaker, Verviers, Belgiu 19 1052 432 show
11 Methods Growth factors/supplements The following reagents were used at indicated concentrations: recombinant 19 541 213 show
12 Methods Antibodies used for flow cytometric analysis and immunoblot analysis Antibodies against the human BM 19 1329 742 show
13 Methods Cell sorting Highly purified CD19+CD27- or CD19+CD27+ cells were obtained by staining CD19+ cells wi 19 233 162 show
14 Methods Western-blot analysis B cells from peripheral blood or cultured cell-lines were lysed in lysis buffe 19 1140 284 show
15 Methods Analysis of BMP-6 messenger RNA (mRNA) expression Endogenous expression of the BMP-6 gene was examin 19 1345 702 show
16 Methods Cell proliferation For estimation of DNA synthesis, CD19+ cells (7.5 × 104 cells/0.2 ml) or Ramos ce 19 538 246 show
17 Methods Determination of cell death Cell death was measured by vital dye exclusion test by staining cells wi 19 275 132 show
18 Methods Statistical analysis The statistical significance of differences between groups was determined using 19 265 92 show