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PMC:1075924 JSONTXT

Divisions (10)
 

div. section text # proj. length # Ann.
0 TIAB Torque-limited RecA polymerization on dsDNA The assembly of RecA onto a torsionally constrained doub 1 925 0 show
1 INTRODUCTION In Escherichia coli, the RecA protein plays a central role in repair of damaged DNA through homologo 1 2731 0 show
2 MATERIALS AND METHODS Magnetic tweezers In the magnetic-tweezers setup used in these experiments (see Figure 1a), a DNA mo 1 678 0 show
3 MATERIALS AND METHODS DNA substrates For assays involving rotationally constrained DNA, pSFV1 (Invitrogen) was cleaved wit 1 1063 0 show
4 MATERIALS AND METHODS Flow cell Polystyrene beads, as well as DNA constructs tethered to a magnetic bead at one end, were 1 377 0 show
5 MATERIALS AND METHODS RecA/DNA reactions The flow cell final volume is ∼100 μl. All reactions were carried out in a buffer 1 491 0 show
6 RESULTS Torsionally unconstrained dsDNA For nicked dsDNA, we observe the expected RecA-induced length extens 1 1316 0 show
7 RESULTS Torsionally constrained dsDNA In the case of torsionally constrained dsDNA, we observed a more compl 1 3257 0 show
8 DATA ANALYSIS AND DISCUSSION When a RecA monomer binds to dsDNA, the base-to-base distance increases by 50% and the helix is unwo 1 15099 0 show
9 CONCLUSION In this paper, we quantitatively described the binding of RecA to torsionally constrained dsDNA. Upo 1 981 0 show