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Divisions (18)
 

div. section text # proj. length # Ann.
0 TIAB Dynamic relocalization of hOGG1 during the cell cycle is disrupted in cells harbouring the hOGG1-Cys 1 1498 0 show
1 INTRODUCTION Oxidative damage to DNA is generated by reactive oxygen species that arise both endogenously and fro 1 4665 0 show
2 MATERIALS AND METHODS Synchronization of HaCat cells, mRNA isolation and northern blot hybridization were carried out as d 1 125 0 show
3 MATERIALS AND METHODS Site-directed mutagenesis of hOGG1 Full-length hOGG1 cDNA cloned into pEGFP-N1 vector (pHOGH1-EGFP-1 1 778 0 show
4 MATERIALS AND METHODS Cell culture, DNA transfection and selection of stable cell lines HeLa S3 cells were cultivated in D 1 809 0 show
5 MATERIALS AND METHODS Nuclear protein extracts, western blot and assay for faPy DNA glycosylase activity Nuclear extracts 1 708 0 show
6 MATERIALS AND METHODS Cell-cycle synchronization, flow cytometry and immunofluorescence HeLa-EGFP-hOGG1 and HeLa-EGFP were 1 1645 0 show
7 MATERIALS AND METHODS Immunostaining and confocal analysis DNA labelling and staining with 5-bromo-2′-deoxyuridine (BrdU l 1 1327 6 show
8 MATERIALS AND METHODS Actinomycin D treatment For actinomycin D treatment, HeLa-EGFP-hOGG1 cells were grown on chamber sli 1 1384 0 show
9 MATERIALS AND METHODS Nuclear matrix preparation Nuclear matrix proteins were fractionated from the indicated cells as des 1 576 0 show
10 RESULTS Cell-cycle-dependent expression of HOGG1 gene The cell-cycle-dependent expression of HOGG1 was follo 1 589 0 show
11 RESULTS hOGG1 is sorted to the nucleoli during S-phase Studies of transiently transfected HeLa cells with th 1 2949 0 show
12 RESULTS The localization of EGFP-hOGG1 to the nucleoli is linked to transcription Actinomycin D specifically 1 1521 0 show
13 RESULTS EGFP-hOGG1-Cys326 is excluded from the nucleolus during S-phase EGFP fused to the C-terminal of hOGG 1 976 0 show
14 RESULTS The associations with interphasic chromatin and nuclear matrix, and with mitotic condensed chromosom 1 1735 0 show
15 RESULTS The EGFP-hOGG1-Glu326 mutant displays a similar cellular distribution to the wild-type protein Since 1 1678 0 show
16 RESULTS EGFP/EYFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 localization during S-phase in live cells O 1 1147 0 show
17 DISCUSSION An increasing number of DNA repair proteins have been shown to have cell-cycle-dependent expression, 1 8035 0 show