> top > docs > PMC:1064895 > divs

PMC:1064895 JSONTXT

Divisions (21)
 

div. section text # proj. length # Ann.
0 TIAB Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor ?B-depend 32 2395 1891 show
1 Introduction Rheumatoid arthritis (RA) is characterized by infiltrations of macrophages and T cells into the join 25 3227 2451 show
2 Materials and methods Patients Informed consent was obtained from 24 patients (5 men and 19 women) with RA who fulfilled t 25 783 527 show
3 Materials and methods Reagents Recombinant IL-17, IL-18, IL-15, monocyte chemoattractant protein-1 (MCP-1), macrophage inf 25 832 641 show
4 Materials and methods Production of IL-17 by T cell receptor activation, cytokines or chemokines PBMC were prepared from h 25 1029 605 show
5 Materials and methods CD4+ T-cell isolation by MACS Anti-CD4 microbeads were used essentially as recommended by the manufa 25 1096 690 show
6 Materials and methods Enzyme-linked immunosorbent assay of IL-17 IL-17 in culture supernatants was measured by sandwich en 25 1480 910 show
7 Materials and methods Quantification of IL-17 mRNA by semiquantitative reverse transcription–polymerase chain reaction PBM 25 1592 972 show
8 Materials and methods Western blot analysis of Akt, phosphorylated Akt and IκB-α PBMC were incubated with anti-CD3 (10 μg/ 25 1256 757 show
9 Materials and methods Gel mobility-shift assay of NF-κB binding site Nuclear proteins were extracted from about 5 × 106 PB 25 1146 764 show
10 Materials and methods Cell viability (Trypan blue dye exclusion assay) For cell viability assays, the trypan blue dye excl 25 394 231 show
11 Materials and methods Statistical analysis Data are expressed as means ± SEM. Statistical analysis was performed with Stud 25 184 93 show
12 Results IL-17 production in PBMC from patients with RA, patients with OA and normal individuals PBMC were se 25 625 503 show
13 Results Increased IL-17 production in PBMC of patients with RA by anti-CD3 and/or anti-CD28, and PHA Because 25 848 692 show
14 Results Regulation of IL-17 production in RA PBMC by inflammatory cytokines and chemokines Because RA PBMC i 25 931 847 show
15 Results Inhibition of IL-17 production by signal transduction inhibitors and anti-rheumatic drugs Having obs 25 1429 1040 show
16 Results IL-17 mRNA expression in RA PBMC To see whether enhanced IL-17 production could be regulated at a tr 25 390 271 show
17 Results Activation of PI3K/Akt signal transduction pathway on IL-17 production by anti-CD3 To determine down 25 829 713 show
18 Results Activation of the NF-κB and activator protein-1 (AP-1) pathway in the IL-17 promoter region To inves 25 1434 1247 show
19 Discussion IL-17 was first described as a T cell product with proinflammatory properties [5,22]. RA is characte 25 6083 4830 show
20 Conclusions We have detected a more pronounced production of IL-17 from RA PBMC in response to IL-15 and MCP-1 a 25 695 536 show